Nataliia O Melnyk
Bogomolets National Medical University, Ukraine
Title: Changers in structure of neurons of Central Nervous System on different experimental models of demyelination and remyelination
Biography
Biography: Nataliia O Melnyk
Abstract
In experimental work was investigating morphological changers of neurons in organs of central nervous system (CNS) in
different experimental models of demyelination and remyelination. After induction of demyelination - EAE (experimental
allergic encephalomyelitis) - in rats, was investigate of changers of neurons in cortex of cerebrum, cerebellum and spinal
cord on 21 days and 39 days. We observed the percentage of neurons with unmodified, moderate and severe structural
changes after staining of histological sections of the brain and spinal cord by toluidine blue and cresyl violet. We studied of
demyelination process of nervous fibers in organs of CNS by the methods of electron microscopy and morphometry. We
observed of remyelination process - the percentage of normal neurons in the brain and spinal cord was increased, the amounts
of neurons with severe and destructive changes were reduce after influence of Rebif® (interferon beta-1a) by 1 and 2 weeks, and
myelinated nerve fibers was regenerate. After induction of demyelination - cuprizone model - in the 129/Sv mice, at 3-5 and 16-
17 months of age we observed changers in structure of neurons and nerve fibers. Cuprizone was provide daily for 3 weeks, in
result, was form demyelination process in CNS. RhLIF was inject after 7-days cuprizone diet, one administration daily, 50 μg/
kg, the hormone of melatonin was provide daily. In the cuprizone-treated mice of both age groups, the percentage of neurons
with severe changes in the brain and spinal cord was increased and after rhLIF and melatonin, the amounts of neurons with
destructive changes were reduce, was less pronounce in aged mice. RhLIF and melatonin may be a perspective neuroprotective drugs.